The study inclusion decision was made on the accident site. The included patients were estimated to develop pre-hospitally significant hypovolaemia (>1000 ml of bleeding). GM6001 ic50 Inclusion criteria were either the actual clinical state or the mechanism of injury (multiple trauma, penetrating trauma of the head, neck, chest or abdomen, fracture of pelvic ring or femur, or a suspicion of injury of large proximal

vessels of the extremities). Patients, who had received more than 500 ml of crystalloids before initial assessment, were excluded. Because of the difficulty to predict the definitive diagnosis and outcome on the accident site, inclusion criteria were selected to be clear and fast to assess to find the patients in the risk of severe hypovolaemia. Not all of them were retrospectively seen as severely injured or hypovolaemic as expected, which can be interpreted from the calculated ISS and RTS-values. The emergency physicians were using a portable clinical blood gas analyzer (i-STAT® by Hewlett-Packard, nowadays a product

of Abbott Laboratories) on the accident site to obtain patients’ blood gas values (pH and BE) and haemoglobin level from the radial or femoral artery. According to the initial base excess (BE) value the patients were stratified into two groups (BE ≤ -3.0 mmol/l or BE > -3.0 mmol/l). In both of these groups the patients were further randomised to receive either fluid resuscitation with 300 mL of hypertonic saline (NaCl 7.5%, HS) or conventional fluid therapy (crystalloids or/and EPZ015938 molecular weight colloids). The infusion type and amount of pre-hospital conventional fluid therapy was decided by the emergency physicians, and was influenced by the levels of shock and transport time. However, the infusion protocol was essentially same in blunt and penetrating trauma patients. Data about the exact

quality and quantity of the conventional fluid therapy was missing from 4 patients, all the other patients received Ringer Acetate (mean 790 ml, range 300-1300) and 7 patients received additional colloid therapy (Plasmafucin or hydroxyethylstarch 6%) (mean 380 ml, range 150-500). Hypertonic saline was administered regardless of the injury mechanism as infusion, which was targeted to end on admission to hospital. Other fluids were interrupted Sclareol while HS was infused. Orion Pharma produced the hypertonic saline solution especially for this study, because at the time of the study hypertonic saline was not yet registered for pharmacological use in Finland. Patient’s blood pressure and heart rate were measured every 10 minutes during transport to the hospital. Blood gas values were measured again on admission to hospital with a subsequent lactate level measurement. Revised Trauma Score (RTS) and Injury TH-302 cost Severity Score (ISS) were calculated retrospectively based on the patients’ pre-hospital notes and the hospital records [11–14]. Data are presented as mean (standard deviation) for continuous and as proportions for discrete values.