The reversal of fluconazole resistance was obtained using
100 μM of the compounds. This concentration did not demonstrate toxicity against human erythrocytes or fungal cells. In conclusion, these compounds could be promising candidates for the reversal of resistance mediated by drug efflux pumps, act synergistically with fluconazole and could serve as prototypes for the synthesis of other molecules that could be capable of inhibiting efflux pumps with greater efficiency. Availability of supporting data The data sets supporting the results of this article Selonsertib are included within the article. Acknowledgments The authors thank FAPERJ (E-26/111.338/2013), FAPESP (2005/59572-7, 2008/55401-1, 2010/17228-6, 2011/03244-2, 2011/11613-8 and 2012/17093-9), CNPq (470360/2012-7) and CAPES for financial support and scholarships. The authors are grateful for the financial and structural support offered by selleck chemicals llc the University of São Paulo through the NAP-CatSinQ (Research Core in Catalysis and Chemical Synthesis). The authors thank also to our lab assistant, Mrs. Geralda
Rodrigues Almeida for her great support and Dr. Louise Kemp for your critical reading of this manuscript. References 1. Brown GD, Meintjes G, Kolls JK, Gray C, Horsnell W, and the Working Group from the EMBO-AIDS Related Mycoses Workshop: AIDS-related mycoses: the way forward. Trends Microbiol 2014, 22(3):107–109.PubMedCrossRef 2. Calton EA, Le Doaré K, Appleby G, Chisholm JC, Sharland M, Ladhani SN, CABIN Participants: Invasive bacterial and fungal infections in paediatric patients with cancer: incidence, risk factors, aetiology and outcomes in a UK regional cohort 2009–2011. Pediatr Blood GDC-0941 nmr cancer 2014, doi:10.1002/pbc. 3. Kauffman CA, Freifeld AG, Andes DR, Baddley JW, Herwaldt L, Walker RC, Alexander BD, Anaissie EJ,
Benedict K, Ito JI, Knapp KM, Lyon GM, Marr KA, Morrison VA, Park BJ, Patterson TF, Schuster MG, Chiller TM, Pappas PG: Endemic fungal infections in solid organ and hematopoietic cell transplant recipients enrolled in the Transplant-Associated Infection Surveillance Network (TRANSNET). Transpl Infect Dis 2014, 0:1–12. 4. Yapar N: Epidemiology and risk factors for invasive candidiasis. Ther Clin Risk Manag 2014, 10:95–105.PubMedCentralPubMedCrossRef 5. Wille MP, Guimarães T, Furtado GHC, Colombo AL: Historical trends in the epidemiology of candidaemia: analysis of an 11-year period Branched chain aminotransferase in a tertiary care hospital in Brazil. Mem Inst Oswaldo Cruz 2013, 108(3):288–292.PubMedCentralCrossRef 6. Odds FC, Brown AJ, Gow NA: Antifungal agents: mechanisms of action. Trends Microbiol 2003, 11:272–279.PubMedCrossRef 7. Martinez L, Falson P: Multidrug resistance ATP-binding cassette membrane transporters as targets for improving oropharyngeal candidiasis treatment. Adv Cell Mol Otolaryngol 2014, 2:1–8.CrossRef 8. Prasad R, Goffeau A: Yeast ATP-binding cassette transporters conferring multidrug resistance. Annu Rev Microbiol 2012, 66:39–63.PubMedCrossRef 9.