parahaemolyticus and 46 non-V. parahaemolyticus bacterial strains templates. The iron-regulated virulence regulatory protein IrgB associated with iron utilization may have profound influences, besides iron acquisition, on the pathogenesis of V. parahaemolyticus (Wong et al., 1996). Therefore, the identification of the irgB gene in V. parahaemolyticus will not only provide a species-specific target for diagnostic application but may also lead to a

better understanding of the genetic mechanisms of its survival in its niche environments as well as its pathogenicity. The detection of tdh and trh genes in V. parahaemolyticus is necessary to determine the real risk posed to human health by the presence of this microorganism. The species-specific irgB gene can be used

as a tool to identify accurately V. parahaemolyticus species by PCR methods, and toxin genes may inform the pathogenic properties of pathogens. Selleckchem Y-27632 Thus, we developed a multiplex PCR assay targeting species-specific marker irgB and toxin genes tdh and trh to detect total and virulent strains of V. parahaemolyticus, which has the potential to reduce V. parahaemolyticus-associated illness in humans. In conclusion, our results demonstrated the successful application of comparative genomics to mine specific markers used in PCR methods for accurate detection and identification of V. parahaemolyticus. The irgB gene was validated as a new V. parahaemolyticus-specific marker. A multiplex PCR assay targeting irgB, selleck chemicals llc tdh and trh genes was successfully developed to detect total and virulent strains of V. parahaemolyticus, which has a potential to be applied in food industries, diagnostics and taxonomic studies. Using this comparative genomics method, it is conceivable the specific targets could be identified for the detection of any bacterium for which Astemizole a genome sequence is available. This work was jointly supported by the grant No. 2009BAK43B31 from the Ministry of Science and Technology of China, the grant Nos. 08391911000, 08142200700 and 08DZ0504200 from Science & Technology Commission of Shanghai Municipality. Table S1. List of 23 CDSs with the lowest e-value ≥0.1 from

Vibrio parahaemolyticus. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. “
“Progress in molecular biology and the advent of rapid and accurate molecular techniques have contributed to precise and rapid detection and differentiation of microbial pathogens. Identification of the Botryosphaeriaceae species based on morphology has been problematic over time. In this study, we used rep-PCR technique as a molecular tool for typing and differentiation of the Botryosphaeriaceae species, well-known and cosmopolitan fungal pathogens on woody plants.