N Engl J Med 2003, 348:1546–1554.PubMedCrossRef 3. Klotz SA, Chasin BS, Powell B, Gaur NK, Lipke PN: Polymicrobial bloodstream infections involving Candida species: analysis of patients and review of the literature. Diagn Microbiol Infect Dis 2007, 59:401–406.PubMedCrossRef

4. Vallés J, Rello J, Ochagavía A, Garnacho J, Alcalá MA: Community-acquired CBL-0137 bloodstream infection in critically ill adult patients: impact of shock and inappropriate antibiotic therapy on survival. Chest 2003, 123:1615–1624.PubMedCrossRef 5. Kumar A, Ellis P, Arabi Y, Roberts D, Light B, Parrillo JE, Dodek P, Wood G, Kumar A, Simon D, Peters C, Ahsan M, Chateau D, Cooperative Antimicrobial Therapy of Septic Shock Database Researc Group: Initiation of inappropriate antimicrobial therapy results in a fivefold reduction of survival in human septic shock. Chest 2009, 136:1237–1248.PubMed 6. Leggieri N, Rida A, François GSK690693 ic50 P, Schrenzel J: Molecular diagnosis of

bloodstream infections: planning to (physically) reach the bedside. Curr Opin Infect Dis 2010, 23:311–319.PubMed 7. Carroll NM, Jaeger EE, Choudhury S, Dunlop AA, Matheson MM, Adamson P, Okhravi N, Lightman S: Detection of and discrimination between Gram-positive and Gram-negative bacteria in intraocular samples by using nested PCR. J Clin Microbiol 2000, 38:1753–1757.PubMedCentralPubMed 8. Didenko VV: DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications. Biotechniques 2001, 31:1106–1121.PubMedCentralPubMed 9. Klaschik S, Lehmann LE, Raadts A, Book M, Hoeft A, Stuber F: Real-time PCR for detection and differentiation of Gram-positive and Gram-negative bacteria. J Clin Microbiol 2002, 40:4304–4307.Tozasertib PubMedCentralPubMedCrossRef 10. Klaschik S, Lehmann LE, Raadts Demeclocycline A, Book M, Gebel J, Hoeft A, Stuber F: Detection and differentiation of in vitro -spiked bacteria by real-time PCR and melting-curve analysis. J Clin Microbiol 2004, 42:512–517.PubMedCentralPubMedCrossRef

11. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Fungal Barcoding Consortium; Fungal Barcoding Consortium Author List: Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci U S A 2012, 109:6241–6246.PubMedCentralPubMedCrossRef 12. Somogyvari F, Serly J, Doczi I, Nagy E: Molecular differentiation of most frequent Candida species causing blood-stream infection. Mycoses 2005, 2:S198. 13. Zhou L, Myers AN, Vandersteen JG, Wang L, Wittwer CT: Closed-tube genotyping with unlabeled oligonucleotide probes and a saturating DNA dye. Clin Chem 2004, 50:1328–1335.PubMedCrossRef 14. Lind K, Ståhlberg A, Zoric N, Kubista M: Combining sequence-specific probes and DNA binding dyes in real-time PCR for specific nucleic acid quantification and melting curve analysis. Biotechniques 2006, 40:315–319.PubMedCrossRef 15.