l. was investigated with an analysis of nuclear ribosomal partial LSU and ITS DNA sequences data by Robledo et al. (2009). In their study, the differentiation of the hyphal system and the basidiospore morphology were outlined as critical features for the definition of genera in the Perenniporia complex. During investigations on wood-inhabiting fungi in China, three undescribed species matching the concepts of Perenniporia were discovered and are introduced. Molecular data can be used to infer relationships amongst groups of morphologically similar basidiomycetes (Yang 2011; Cao

et al. 2012; He and Dai 2012). The aims of this study are to 1) confirm the taxonomic affinity of the new species and 2) infer the evolutionary relationships among representative SCH 900776 in vitro species of Perenniporia selleck to establish if the genus is mono- or polyphyletic. Materials and methods Morphological studies The studied specimens were deposited at the herbaria of the Institute of Microbiology, Repotrectinib in vitro Beijing Forestry University (BJFC) and the Institute of Applied Ecology, Chinese Academy of Sciences (IFP). The microscopic routine followed Dai (2010b). Sections were studied at magnification up to ×1000 using a Nikon Eclipse E 80i microscope and phase contrast

illumination. Drawings were made with the aid of a drawing tube. Microscopic features, measurements and drawings were made from slide preparations stained with Cotton Blue and Melzer’s reagent. Spores

were measured from sections cut from the tubes. In presenting the variation in the size of the spores, 5 % of measurements were excluded from each end of the range, and were given in parentheses. In the text the following abbreviations were used: IKI = Melzer’s reagent, IKI– = negative in Melzer’s reagent, KOH = 5 % potassium hydroxide, CB = Cotton Blue, CB+ = cyanophilous, L = mean spore length (arithmetic average of all spores), W = mean spore width (arithmetic average of all spores), Q = variation in the L/W ratios between the specimens studied, n = number of spores measured from given number of specimens. Special color terms followed Petersen (1996). Molecular study and phylogenetic Clomifene analysis Molecular techniques followed Cui et al. (2008) and Dai et al. (2010). The fungal taxa used in this study are listed in Table 1. Phire Plant Direct PCR Kit (Finnzymes) procedure was used to extract total genomic DNA from the fruitbodies and for the polymerase chain reaction (PCR). DNA sequencing was performed at Beijing Genomics Institute. All newly generated sequences were submitted to GenBank and are listed in Table 1. In the study, sequence data of nuclear ribosomal RNA regions were used to determine the phylogenetic positions of the new species. The internal transcribed spacer (ITS) regions were amplified with the primers ITS4 and ITS5 (White et al. 1990), and the large subunit (nLSU) with the primers LR0R and LR7 (Pinruan et al. 2010).