g ST23, and strains that primarily

affect fish, e g ST2

g. ST23, and strains that primarily

affect fish, e.g. ST260 and ST261, may provide insight into host-adaptation of S. agalactiae. Epidemiological studies are needed to provide insight into the likelihood and routes of interspecies transmission of strains that are associated with fish, sea mammals and invasive disease in humans as well as control measures needed to prevent transmission and disease. Acknowledgements This work was supported by a joint PhD grant from the University of Stirling and the Moredun Research Institute. We acknowledge the following individuals for providing the fish and frog isolates used in this study: Hugh W Ferguson, School of Veterinary Medicine, St. George’s University, Grenada, W. Indies; Carlos Iregui, Laboratorio de Patología, Facultad de Medicina y de Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia; C188-9 concentration Terutoyo Yoshida, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan; Temdoung Somsiri, Aquatic Animal Health Research Institute, Kasetsart University Campus, Jatujak, Bangkok, Thailand; Janenuj Wongtavatchai, Department of Medicine, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand; Francois Lieffrig

Centre d’ Economie Rurale Groupe, Marloie, Belgium; Jeremy Carson, Fish Health Unit of the Tasmanian Aquaculture and Fisheries Institute, University of Tasmania, Australia; Nicky Buller, Department of Agriculture and Food Western Australia, South Perth, Australia. We also thank Pharmaq AS Norway for their support in collecting one of the strains, Ian Heron for excellent technical assistance, and Nicola Jones for assignment of novel alleles and ST numbers. The Scottish PARP signaling Strandings Scheme receives not financial support from the Scottish Government Marine Directorate and the UK Department of Environment, Farming and Rural Affairs (Defra). References 1. Manning SD, Springman AC, Lehotzky E, Lewis

MA, Whittam TS, Davies HD: Multilocus sequence types associated with neonatal group B streptococcal sepsis and meningitis in Canada. J Clin Microbiol 2009, 47:1143–1148.PubMedCrossRef 2. Phares CR, Lynfield R, Farley MM, Mohle-Boetani J, Harrison LH, Petit S, et al.: Epidemiology of invasive group B streptococcal disease in the United States, 1999–2005. J Am Med Assoc 2008, 299:2056–2065.CrossRef 3. Chaiwarith R, Jullaket W, Bunchoo M, Nuntachit N, Sirisanthana T, Supparatpinyo K: Streptococcus agalactiae in adults at Chiang Mai University Hospital: a retrospective study. BMC Infect Dis 2011, 11:149.PubMedCrossRef 4. Lambertsen L, Ekelund K, Skovsted IC, Liboriussen A, Slotved HC: Characterisation of invasive group B streptococci from adults in Denmark 1999 to 2004. Eur J Clin Microbiol Infect Dis 2010, 29:1071–1077.PubMedCrossRef 5. Skoff TH, Farley MM, Petit S, Craig AS, Schaffner W, Gershman K, et al.: Increasing burden of invasive group B streptococcal disease in nonpregnant adults, 1990–2007. Clin Infect Dis 2009, 49:85–92.

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