Briefly, media samples were mixed with 0.5 mL 90:10 methanol/1 N NaOH (pH 10). NOR is pinkish at this pH, which allows for spectrophotometric measurement at 595 nm with a 96-well Tecan plate reader. Statistical analyses All experiments were conducted with at least 3 replicates and statistical significance

was Tideglusib research buy evaluated using Student’s t-tests. Acknowledgments The authors thank Fen Yang for early protocol development, and Lixin Duan and Zhen Xue at the Key Laboratory of Molecular Plant Physiology, CAS, for technical assistance. This research was supported by the Key Innovation Project (KSCX2-YW-N-033) and 100-Talent Project of the Chinese Academy of Sciences, granted to CML. Electronic supplementary material Additional file 1: Structures of D-glucose, D-glucal and D-galactal. (PPTX 55 KB) Additional file 2: Table S1: Primers used for qRT-PCR. (PPTX 71 KB) References 1. Yu J, Cleveland TE, Nierman WC, Bennett

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