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“BACKGROUND
Although 4SC-202 adverse events are uncommon with hyaluronic acid (HA) fillers, the use of hyaluronidase permits the reversal of treatment complications or overcorrection.
OBJECTIVE
This study sought
to determine an in vitro dose-response relationship between ovine testicular hyaluronidase (OTH) and three HA dermal fillers (24-mg/mL smooth gel, 20-mg/mL particulate gel, and 5.5-mg/mL particulate gel with 0.3% lidocaine).
METHODS AND MATERIALS
The dose response of each was measured after incubation for 30 minutes in concentrations ranging between 5 and 40 U of OTH. Timed responses for the 24-mg/mL and 20-mg/mL HA fillers were obtained after incubation with 20 U of OTH for 15 to 120 minutes.
RESULTS
After all dose responses and timed-interval tests, the 24-mg/mL HA smooth gel filler exhibited more resistance against in vitro enzymatic degradation to OTH than the 20- and 5.5-mg/mL HA particulate gels.
CONCLUSION
This resistance to degradation in vitro may be attributed to the higher HA content of the 24-mg/mL HA smooth gel, the degree of crosslinking, and the cohesive property of the gel filler.
This study was funded by a grant from Allergan, Inc., Santa Barbara, CA. Derek Jones, MD, is
a consultant, investigator, advisory board member, and speaker for Allergan, Inc. He received no compensation for this study. Drs. Tezel and Borrell selleck chemicals are employed by Allergan, Inc., Santa Barbara, CA. Editorial assistance was provided by Health Learning Systems, a part of CommonHealth, Parsippany, NJ.”
“Assisted phytoextraction is the application of chelators into soil to enhance plant tolerance to heavy metals. During this process, water-soluble complexes of chelators and toxic elements
are formed. This contributes to increased leaching of pollutants from soil into groundwater. The aim of the study was to determine the extent of leaching of mercury from soil during chemically assisted phytoextraction. The process was enhanced by use of EDTA, KI and citric Selleck MCC950 acid in different concentrations. The leaching of mercury from contaminated soil was in the range 39.444.1% depending on the time and the concentration of mercury. Application of each of the chelators used during the experiment increased the leaching of mercury from soil. The highest Hg leaching was observed for the process in which EDTA was used as a soil amendment. Leaching of mercury from contaminated soil was dependent on both mercury and chelator concentration. The higher the chelator concentration in soil, the greater the mercury leaching. The results of the study showed that the lowest Hg leaching was observed in the process in which citric acid was applied to the soil. Therefore, use of this compound as a chelator during assisted phytoextraction of Hg contaminated soil should be recommended.