20 All the data was presented as mean ± S.E.M and analyzed by paired-t-test using SPSS software package (SPSS, Cary, NC, USA). The present investigation highlights the antidiabetogenic and antioxidant efficacy of C. attenuata extract. The antidiabetic potency has been evaluated by the measurement of parameters like body weight, water and fluid intake, fasting blood glucose level, intravenous glucose tolerance along with serum insulin level. It was concluded that there was a significant decrease (p < 0.01) in body weight, food and liquid intake of diabetic group as compared to the control group.

After administration of CAEt there was a significant recovery of these parameters toward the control level. Treatment of CAEt to streptozotocin diabetic animals resulted in a complete

recovery of fasting blood glucose level and the animals were able A-1210477 clinical trial to tolerate the exogenous glucose load compared with normal controls ( Table 1). There was a significant increase in blood glucose level (p < 0.05) in diabetic rats when compared with normal controls. CAEt also showed significant reduction (p < 0.01) in serum glucose level in STZ diabetic rats ( Table 1). The antioxidant efficacy was, contrary, based on the measurement of free radical scavenging enzymes viz. TBARS, GSH, GSH-R, SOD and CAT. Table 2 shows the levels of TBARS, GSH and GSH-R in see more liver and kidney of control either and experimental animals (p < 0.001). A significant elevation in tissues TBARS and significant reduction in GSH, and GSH-R was observed in the diabetic control rats as compared to the normal control rats. Oral administration of CAEt (100 and 250 mg/kg bw) for three weeks shows significant reduction in TBARS and increase in GSH-R in both liver and kidney (p < 0.001). With respect to GSH there was a significant increase in the glutathione in the liver and kidney. Table 2 also cite the activities of the enzymatic antioxidants

SOD and CAT in liver and kidney (p < 0.001). Activities of these enzymes decreased significantly in the diabetic control rats as compared to the normal control (p < 0.001). Oral administration of CAEt (100 mg and 250 mg/kg) for 3 weeks significantly reversed these enzymes to near normal values. The various parameters of blood lipid profile of severely diabetic rats were tested before and after treatment. The effect of CAEt 100 and 250 mg/kg on TC, TG and LDL levels are shown in Table 2. A significant increase in TG (p < 0.01), TC (p < 0.05) and LDL (p < 0.05) levels was observed in diabetic controls as compared to normal controls. Treatment by CAEt significantly reduced TC (p < 0.05), TG (p < 0.01), LDL (p < 0.05), free fatty acids (p < 0.05) and phospholipids (p < 0.05) levels as well as significantly increased HDL levels. Following hypothesis has been proposed for the mode of action of the C. attenuata extract.