1 to -2.5, P = 0.003). Collagen content in ACLT and contralateral cartilage were similar. PG content was reduced and collagen orientation angle was increased in the superficial tissue of ACLT cartilage compared to the contralateral cartilage.

Conclusions: We found the novel result that chondrocyte deformation behavior in the superficial tissue of rabbit articular cartilage is altered already at 4 weeks after ACLT, likely because of changes in collagen fibril orientation and a reduction in PG content. (C) 2012 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.”
“Purpose: To evaluate the diagnostic accuracy of the atopy patch test (APT) in detection

of delayed-type sensitization to different food allergens, in children with digestive symptoms and learn more with/or without cow’s milk allergy.

Material and methods: 28 children suspected of food allergy-related gastrointestinal symptoms were underwent diagnostic procedures as skin prick test (SPT), specific immunoglobulin E (sIgE), APT, oral

food challenge and then divided into two groups according to the results of oral food challenge: A – 17 children with cow’s milk allergy, B – 11 children without cow’s milk allergy. Sensitivity, specificity, positive (PPV) and negative (NPV) predictive values of APT were calculated.

Results: A positive challenge response to milk was found in 60,7% of examined children. No statistical difference in the AZ 628 research buy prevalence of positive SPT and

sIgE to milk has been found between the groups. The prevalence of positive APT to other food allergens was significantly higher in children PF-6463922 with allergy to milk (p<0,005). No statistical difference of positive APT to other food allergens has been found in children with/or without cow’s milk allergy. APT sensitivity and specificity in children with cow’s milk allergy obtained on 77%/73%; where PPV obtained on 71%.

Conclusions: High sensitivity and specificity of APT confirm its accuracy in diagnostics of delayed-type reactions in children with gastrointestinal symptoms. Combined atopy patch test and oral food challenge is essential for avoidance of unnecessary elimination diet. Delayed-type sensitization to other food allergens is common in children with cow’s milk allergy.”
“Objective: Chondrocyte hypertrophy followed by cartilage destruction is a crucial step for osteoarthritis (OA) development, however, the underlying mechanism remains largely unknown. The objectives of this study are to identify the gene that may cause cartilage hypertrophy and to elucidate its role on OA pathogenesis.

Design: Gene expression profiles of cartilages from OA patients and normal subjects were examined by microarray analysis. Expression of deiodinases, enzymes for regulation of triiodothyronine (T3) biosynthesis, in human and rat articular cartilage (AC) were examined by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR).