0-5.0% (w/v) NaCl (optimum of 2.0% NaCl). Under aerobic conditions, the major isoprenoid quinones were ubiquinone-9 and menaquinone-9 and the minor quinones were ubiquinone-8 and menaquinone-8. The major cellular fatty acids were C-18:1 omega 7c, C-16:1 omega 7c and C-16:0 and the

hydroxy acids were C-10:0 3-OH and C-12:0 3-OH. The DNA G+C content was 48.3-48.7 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the isolates within the radiation of the genus Endozoicomonas in a broad clade of uncultured clones recovered from various marine invertebrates. The isolates exhibited 96.5-96.9 % 16S rRNA gene sequence similarity with Endozoicomonas elysicola MKT110(T) and Endozoicomonas Selleckchem CT99021 montiporae CL-33(T), with which the isolates formed a monophyletic cluster with 100 % bootstrap support. The phenotypic features (carbohydrate fermentation, quinone system and some major cellular fatty acids) differed from those of members of the genus Endozoicomonas, which are aerobic, produce little PLX4032 chemical structure or no menaquinone under aerobic conditions and possess different amounts of C-14:0 and C-18:1 omega 7c. Although some phenotypic differences were identified, the isolates should be assigned to the genus Endozoicomonas on the basis of congruity of phylogeny and should be classified as representatives of a novel species, for which the name Endozoicomonas numazuensis sp. nov. is proposed.

The type strain is HC50(T) (=NBRC 108893(T) =DSM 25634(T)). An emended description of the genus Endozoicomonas is presented.”
“The

paper aims at developing a simple two-step homogenization scheme for prediction of elastic properties of a high performance concrete (HPC) in which microstructural heterogeneities are distinguished with the help of nanoindentation. The main components of the analyzed material include blended cement, CP-868596 purchase fly-ash and fine aggregate. The material heterogeneity appears on several length scales as well as porosity that is accounted for in the model. Grid nanoindentation is applied as a fundamental source of elastic properties of individual microstructural phases in which subsequent statistical evaluation and deconvolution of phase properties are employed. The multilevel porosity is evaluated from combined sources, namely mercury intrusion porosimetry and optical image analyses. Micromechanical data serve as input parameters for analytical (Mori-Tanaka) and numerical FFT-based elastic homogenizations at microscale. Both schemes give similar results and justify the isotropic character of the material. The elastic stiffness matrices are derived from individual phase properties and directly from the grid nanoindentation data with very good agreement. The second material level, which accounts for large air porosity and aggregate, is treated with analytical homogenization to predict the overall composite properties. The results are compared with macroscopic experimental measurements received from static and dynamic tests.