Stromata were nearly dry at collection times and may be more reddish PLX-4720 cell line brown when fresh, as suggested
by the red colour after rehydration. Dry stromata may be confounded with those of H. neorufa and H. neorufoides, which differ in a yellow colour when young, in darker and more compact dry stromata, in yellow perithecial walls, and in many culture and anamorph characteristics. The dark brown dry stromata of H. petersenii lack violet tones. T. petersenii sporulates well on all media, grows well at 30°C and grows substantially faster on all media than T. subeffusum. The large coilings on the surface of larger colonies of T. subeffusum on CMD close to the distal margin have been detected in all isolates. They have not been seen in any other Hypocrea anamorphs in Europe so far, i.e. they are characteristic for this species. In addition, T. subeffusum is one of the few species that sporulate well on CMD, but poorly on SNA. Hypocrea valdunensis Jaklitsch, sp. nov. Fig. 24 Fig. 24 Teleomorph of Hypocrea valdunensis (WU 29516). a–c. Fresh stromata. d–k. Dry stromata (d–f. immature). l. Rehydrated mature stroma. m. Stroma in 3% KOH after rehydration. n. Rehydrated stroma surface showing ostiolar openings and inhomogeneous pigment. o. Perithecium in section. p. Cortical and subcortical tissue in section.
FDA approved Drug Library concentration q. Subperithecial tissue in section. r. Stroma base in section. s. Ascus with ascospores in cotton blue/lactic acid. t, u. Hairs on stroma surface. v. Tubercular stroma surface in section. w. Stroma surface in face view. Scale pentoxifylline bars: a–c = 2 mm. d, h, j, k = 1 mm. e, f, i, m = 0.3 mm. g = 0.2 mm. l = 0.7 mm. n = 70 μm. o, r, v = 25 μm. p, q, t, u = 15 μm. s, w = 10 μm MycoBank MB 5166708 Anamorph:
Trichoderma valdunense Jaklitsch, sp. nov. Fig. 25 Fig. 25 Cultures and anamorph of Hypocrea valdunensis (CBS 120923). a–c. Cultures (a. on CMD, 19 days; b. on PDA, 19 days; c. on SNA, 21 days). d, e. Conidiation tufts (CMD; d. 27 days, stereo-microscope. e. 11 days, compound microscope, 10× SU5402 ic50 objective). f–m. Conidiophores (f–h, k–m. CMD, 6–8 days; i, j. MEA, 10 days). n. Phialides (CMD, 8 days). o–r. Conidia (o. MEA, 10 days; p. from tuft, CMD, 27 days; q, r. CMD, 6 days). a–r. All at 25°C. Scale bars: a–c = 15 mm. d, e. = 80 μm. f, g = 20 μm. h–k = 15 μm. l–n = 10 μm. o, p = 5 μm. q, r = 3 μm MycoBank MB 5166709 Differt a Hypocrea viridescente ascosporis minoribus, incremento tardiore et conidiis glabris. Ascosporae bicellulares, hyalinae, verruculosae vel spinulosae, ad septum disarticulatae, pars distalis (sub)globosa, (3.0–)3.3–3.7(–4.0) × (2.8–)3.0–3.5 μm, pars proxima oblonga, (3.5–)3.8–4.5(–5.0) × (2.3–)2.5–3.0 μm. Phialides divergentes, lageniformes, (4.5–)6–11(–14) × (1.8–)2.2–2.8(–3.2) μm. Conidia ellipsoidea vel ovalia, luteo-viridia in agaro CMD, glabra, (2.7–)3.2–3.8(–4.0) × (2.3–)2.5–2.8(–3.0) μm.