, Madison, WI). Alignment analysis was also performed between the identified epitopes and other associated flavivirus strains, including the members of JEV serocomplex, and another three antigenically related flaviviruses, DENV (type 1-4), YFV and TBEV, the factors of isolation time and geographical location of all strains were considered. Acknowledgements The authors thank Dr. Peter Wilker for editing the manuscript. This study was supported by National High-Tech Research and Development Program of China (No. 2011AA10A212) and

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