For each analyte, the recorded peak position and the relative intensities in the recorded spectra were independent of the preparation method used to produce silver colloids. All investigated analytes adsorbed on the three classes of silver

colloids gave comparable scattering intensities, indicating RXDX-101 ic50 that the PEG-reduced silver colloid provides comparable SERS enhancement as conventional colloids. Conclusions In this paper, we propose an easy, fast, one-step, facile, and green method for the synthesis of silver nanoparticles thus improving the straightforward creation of functionalized nanoparticles for biomedical usage. No toxic reagents, surfactant, and organic or inorganic solvents were implicated in the entire chemical trial. The successfully synthesized silver nanoparticles, which were produced using PEG

200 as reducing and stabilizing agents, own SERS-active properties. Though the procedure requires boiling conditions, the success of the experiment stands out throughout the speed in which biological clean nanoparticle systems can be synthesized in order to use them subsequently in analytical and biomedical applications. The major finding of this fast, one-step synthesis method resides in the use of additional -OH groups that are generated in the solution by sodium hydroxide, RG7420 clinical trial enhancing the speed of the chemical reduction of silver ions. The as-prepared PEG-coated silver nanoparticles showed a great stability in time. Acknowledgments This research was supported by CNCSIS-UEFISCDU, project number

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